Hemodialysis restored iron distribution that was sequestered in the spleen by bilateral nephrectomy.

نویسندگان

  • Aritoshi Kida
  • Takahiro Kuragano
  • Minoru Furuta
  • Yoshinaga Otaki
  • Yukiko Hasuike
  • Saori Matsuda
  • Nobuhide Akaike
  • Yukifumi Kokuba
  • Takeshi Nakanishi
چکیده

Acute kidney injury (AKI) is associated with dysregulated iron metabolism, which may play a significant role in cellular injury. The effect of hemodialysis (HD) on iron metabolism in AKI therapy has not been well defined. The effects of HD on iron parameters were tested in control rats and bilateral nephrectomy (BNx) rats. The BNx rats were divided into the following three groups: 1) the sham-operated group (BNx-Sham), 2) the BNx group, and 3) the HD group (BNx-HD), which received HD therapy 40-45 h after BNx. Sections of the liver or spleen were stained with Berlin blue to examine the accumulation of iron. The mRNA levels of hepcidin and ferroportin 1 in the spleen and liver were also quantified using RT-PCR. In the BNx group, the plasma iron and hematocrit levels were decreased, and hepcidin levels were increased. The iron staining in the spleen in the BNx group was significantly more intense than that in the BNx-Sham group; however, after an HD session, splenic iron staining diminished to the level of the sham group along with an increase in plasma iron and a decrease in hepcidin. BNx moved iron from hemoglobin and the plasma to the spleen, which is associated with an increase in plasma hepcidin. A single HD session accelerated the release of iron from the spleen, and the increased plasma iron was linked to the removal of hepcidin. Our data suggested that hepcidin might dynamically modulate the iron metabolism in BNx as well as in HD.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Treatment of patients with metastatic renal cell carcinoma undergoing hemodialysis: case report of two patients and short literature review

BACKGROUND Renal cell carcinoma (RCC) may involve both kidneys. When bilateral nephrectomy is necessary renal replacement therapy is mandatory. Treating such patients with sequential therapy based on cytokines, antiangiogenic factors and mammalian target of rapamycin (mTOR) inhibitors is challenging. CASE PRESENTATION The first case, a 50-year-old Caucasian female, underwent a radical right n...

متن کامل

A Hemodynamic Evaluation of Bilateral Nephrectomy and Hemodialysis in Hypertensive Man By CONSTANTINE

Three hypertensive men with end-stage primary renal disease were hemodynamically evaluated before and 3 to 4 weeks after bilateral nephrectomy and before and after 6 hours of hemodialysis with the twin coil artificial kidney. Conclusions include the following: (1) The height of the blood pressure and changes in plasma volume (P.V.) are not necessarily directly related in the early renoprival st...

متن کامل

Bilateral Synchronous Sporadic Renal Cell Carcinoma: Retroperitoneoscopic Strategies and Intermediate Outcomes of 60 Patients

OBJECTIVE To evaluate the presentation, management, pathology, and functional and oncological outcomes of patients undergoing retroperitoneoscopic treatment of bilateral synchronous sporadic RCC at our institution. METHODS We retrospectively evaluated the records of 60 patients with bilateral synchronous sporadic RCC who underwent retroperitoneoscopic treatment at the General Hospital of Peop...

متن کامل

Evidence for an extrarenal source of inactive renin in rats.

We studied the source of inactive renin in plasma by investigating the changes of active and inactive renin after bilateral nephrectomy in the rat. Active renin rapidly decreased after bilateral nephrectomy, with a half-life of approximately 15 minutes. Inactive renin, on the other hand, was 20.96 +/- 1.63 ng/ml/hr before nephrectomy and gradually increased to reach a peak at 20 hours after nep...

متن کامل

A hemodynamic evaluation of bilateral nephrectomy and hemodialysis in hypertensive man.

Three hypertensive men with end-stage primary renal disease were hemodynamically evaluated before and 3 to 4 weeks after bilateral nephrectomy and before and after 6 hours of hemodialysis with the twin coil artificial kidney. Conclusions include the following: (1) The height of the blood pressure and changes in plasma volume (P.V.) are not necessarily directly related in the early renoprival st...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • American journal of physiology. Renal physiology

دوره 306 12  شماره 

صفحات  -

تاریخ انتشار 2014